RAT ANAL ALLOTRANSPLANTATION MODEL UTILIZING SUPER-MICROSURGERY

Jun Araki¹, Makoto Mihara¹, Takuya Iida¹, Isao Koshima¹, Joji Kitayama², Tomoyuki Sato³.

¹Department of Plastic Surgery, University of Tokyo, Tokyo, Japan; ²Department of Surgical Oncology & Vascular Surgery, University of Tokyo, Tokyo, Japan; ³Saitama Shinkaibashi Crinic, Saitama, Japan.

Intriduction: Since its development in 1908, Ostomy has served as an effective surgery for various anorectal disfunctions. However it must also be noted that those patients suffered greatly from stresses caused by their stoma. Many alternative therapies have been examined and developed, but none have been able to solve this critical issue. Meanwhile, due to the improvements in operational methods and immunosuppressive therapy, allotranplantation: especially composite tissue allotransplantation for QOL of patients such as faces and hand transplantation, has gained great popularity in recent years. Therefore, we started in our development of the anal transplantation model.

Methods: Anal transplantation was performed to six adult wistar rats divided into 2 groups. Group 1 was with vascular anastomoses, while group 2 was without.

Results: The grafts in group 1 survived and were able to fully recover their anal function. However, many of the grafts in group 2 did not survive, and those that did had major defecations in their anus thus never recovering their anal function.

Conclusions: We succeeded in establishing the rat anal transplantation model utilizing Super-microsurgery. While research in anal transplantation was behind compared to those in other fields of study, we hope that this model will bring significant possibilities for the future.